Zhongguo shi yan xue ye xue za zhi · 2023
[Molecular Mechanism of Busulfan Inhibiting the Malignant Biological Characteristics of Multiple Myeloma Cells].
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Huang Tao, Yin Yue, Luo Yun-Long, Lin Fang-Heng, Liu De-Bin
Аннотация
OBJECTIVE: To investigate the effect of Busulfan on the malignant biological characteristics of multiple myeloma cells and explore the molecular mechanism.
METHODS: Multiple myeloma RPMI8226 cells were treated with Busulfan at different concentrations. Cell proliferation activity was detected by MMT assay, and cell apoptosis was detected by flow cytometry with Annexin V/PI double staining. Real-time quantitative PCR was used to detect the mRNA expression of apoptotic regulatory molecules Bax、Bcl-2 and Wnt3a/β-catenin pathway, and Western blot was used to detect the expression changes of Bax, Bcl-2 and Wnt3a/β-catenin pathway protein.
RESULTS: Busulfan inhibited cell proliferation and induced apoptosis of myeloma RPMI8226 cells (P<0.05). After treatment with Busulfan at different concentrations for 48 h, the expression of anti-apoptotic protein Bcl-2 was decreased, the expression of pro-apoptotic protein Bax was up-regulated, and the activation of Wnt3a/β-catenin signaling pathway was inhibited to induce programmed death of RPMI8226 cells (P<0.05).
CONCLUSION: Busulfan can inhibit the malignant biological characteristics of myeloma RPMI 8226 cells, and its mechanism may be related to regulating the expression of Bcl-2 family proteins and inhibiting the activation of Wnt3a/β-catenin signaling pathway.
METHODS: Multiple myeloma RPMI8226 cells were treated with Busulfan at different concentrations. Cell proliferation activity was detected by MMT assay, and cell apoptosis was detected by flow cytometry with Annexin V/PI double staining. Real-time quantitative PCR was used to detect the mRNA expression of apoptotic regulatory molecules Bax、Bcl-2 and Wnt3a/β-catenin pathway, and Western blot was used to detect the expression changes of Bax, Bcl-2 and Wnt3a/β-catenin pathway protein.
RESULTS: Busulfan inhibited cell proliferation and induced apoptosis of myeloma RPMI8226 cells (P<0.05). After treatment with Busulfan at different concentrations for 48 h, the expression of anti-apoptotic protein Bcl-2 was decreased, the expression of pro-apoptotic protein Bax was up-regulated, and the activation of Wnt3a/β-catenin signaling pathway was inhibited to induce programmed death of RPMI8226 cells (P<0.05).
CONCLUSION: Busulfan can inhibit the malignant biological characteristics of myeloma RPMI 8226 cells, and its mechanism may be related to regulating the expression of Bcl-2 family proteins and inhibiting the activation of Wnt3a/β-catenin signaling pathway.