Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2008
[Expression of periostin and the effect of hydrocortisone on it in human fibroblasts of scar].
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Song Zhen Hua, Qin Ze Lian
Аннотация
OBJECTIVE: To probe into the role of periostin in the formation of scars and investigate its reaction to hydrocortisone.
METHODS: Primary fibroblasts were cultured in vitro, then RT-PCR and immunocytochemical technique were used to examine the expressions of mRNA and preotein of periostin respectively in 24 samples of keloid fibroblasts (KFb), hypertrophic scar fibroblasts (HFb) and normal skin fibroblasts (SFb).
RESULTS: The mRNA levels of periostin in KFb (1.645+/-0.549) and HFb (1.084+/-0.396) were both higher than that in SFb (0.274+/-0.215, P<0.05). After hydrocortisone was added, the mRNA levels of periostin in KFb and HFb were both decreased by 32% and 47% respectively (P<0.05 ); The protein of periostin distributed mainly in the endochylema around the nucleus of fibroblasts. The protein levels of periostin in KFb (91.815+/-0.961) and HFb (70.166+/-2.250) were both higher than that in SFb (41.011 +/- 1.576, P<0.01).
CONCLUSION: The expressions of periostin were increased in the fibroblasts of hyperplasic scars, which could be inhibited by hydrocortisone. periostin may affect the function of fibroblasts of hyperplasic scars. periostin may be a cicatrix specific gene.
METHODS: Primary fibroblasts were cultured in vitro, then RT-PCR and immunocytochemical technique were used to examine the expressions of mRNA and preotein of periostin respectively in 24 samples of keloid fibroblasts (KFb), hypertrophic scar fibroblasts (HFb) and normal skin fibroblasts (SFb).
RESULTS: The mRNA levels of periostin in KFb (1.645+/-0.549) and HFb (1.084+/-0.396) were both higher than that in SFb (0.274+/-0.215, P<0.05). After hydrocortisone was added, the mRNA levels of periostin in KFb and HFb were both decreased by 32% and 47% respectively (P<0.05 ); The protein of periostin distributed mainly in the endochylema around the nucleus of fibroblasts. The protein levels of periostin in KFb (91.815+/-0.961) and HFb (70.166+/-2.250) were both higher than that in SFb (41.011 +/- 1.576, P<0.01).
CONCLUSION: The expressions of periostin were increased in the fibroblasts of hyperplasic scars, which could be inhibited by hydrocortisone. periostin may affect the function of fibroblasts of hyperplasic scars. periostin may be a cicatrix specific gene.