Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery · 2005
[The influence of adrenaline on the expression of TGF-beta1, bFGF and I procollagen for hypertrophic scar].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Zhang Cheng-de, Tian Ying, Song Lan, Zhang Cai-ping
Аннотация
OBJECTIVE: To investigate the influence of adrenaline on the expression of TGFbeta1, bFGF and procollagen for human normal and hypertrophic scar dermal fibroblasts cultured in vitro.
METHODS: Human normal and hypertrophic scar dermal fibroblasts were propagated in a serum-free in vitro model with adrenaline for 24 hours. The human mRNA levels of bFGF, TGF-beta1 and I procollagen in fibroblasts were determined by RT-PCR. Levels of bFGF and TGF-beta1 in the supernatants of fibroblasts cultured in vitro were determined by enzyme-linked immunosorbent assay (ELISA).
RESULTS: In our study, adrenaline caused statistically significant increase in the peak levels of bFGF for normal and hypertrophic scar fibroblast cell lines (P < 0.01). It also caused statistically significant decrease in the level of TGF-beta1 for normal and hypertrophic scar fibroblast cell lines. Modulation of normal fibroblasts with 0.05, 0.10 and 0.20 micromol/L adrenaline resulted in a statistically significant (P < 0.01) decrease in the expression of I procollagen mRNA. However, only 0.20 micromol/L adrenaline can decreased the mRNA expression of I procollagen in the hypertrophic scar fibroblasts.
CONCLUSIONS: We conclude from these results that adrenaline can increase the production of bFGF and decrease production of TGF-beta1 and I procollagen in human normal dermal and hypertrophic scar fibroblasts cultured in vitro.
METHODS: Human normal and hypertrophic scar dermal fibroblasts were propagated in a serum-free in vitro model with adrenaline for 24 hours. The human mRNA levels of bFGF, TGF-beta1 and I procollagen in fibroblasts were determined by RT-PCR. Levels of bFGF and TGF-beta1 in the supernatants of fibroblasts cultured in vitro were determined by enzyme-linked immunosorbent assay (ELISA).
RESULTS: In our study, adrenaline caused statistically significant increase in the peak levels of bFGF for normal and hypertrophic scar fibroblast cell lines (P < 0.01). It also caused statistically significant decrease in the level of TGF-beta1 for normal and hypertrophic scar fibroblast cell lines. Modulation of normal fibroblasts with 0.05, 0.10 and 0.20 micromol/L adrenaline resulted in a statistically significant (P < 0.01) decrease in the expression of I procollagen mRNA. However, only 0.20 micromol/L adrenaline can decreased the mRNA expression of I procollagen in the hypertrophic scar fibroblasts.
CONCLUSIONS: We conclude from these results that adrenaline can increase the production of bFGF and decrease production of TGF-beta1 and I procollagen in human normal dermal and hypertrophic scar fibroblasts cultured in vitro.