Zhurnal mikrobiologii, epidemiologii i immunobiologii · 2014
[Features of resistance formation to beta-lactam antibiotics in Francisella tularensis subsp. mediasiatica].
СтатьяНазвание и аннотация: Оригинал на русском
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Tsimbalistova M V, Pavlovich N V
Аннотация
AIM: Determination of penicillin resistance features in tularemia causative agents of the mediasiatica subspecies, stability evaluation of differences in strains of various taxa and development of a rapid method of F. tularensis intraspecies differentiation.
MATERIALS AND METHODS: Beta-lactamase activity was determined in 30 strains of Francisella genus bacteria by quantitative iodometric method.
RESULTS: All the strains regardless of subspecies membership were characterized by high resistance to beta-lactam antibiotics, and bacteria of the mediasiatica subspecies in contrast to other francisella did not synthesize beta-lactamase. Our attempts to induce beta-lactamase activity in vitro and in vivo in strains of this subspecies did not succeed. A method of intraspecies differentiation of F. tularensis by nitorcefin disks is proposed based on the distinctive feature.
CONCLUSION: A high level of F. tularensis subsp. mediasiatica resistance to beta-lactams in vitro and their inefficiency during therapy of experimental tularemia due to a beta-lactamase negative strain suggests that F. tularensis beta-lactamase is not the leading factor in formation of native penicillin resistance of tularemia causative agent.
MATERIALS AND METHODS: Beta-lactamase activity was determined in 30 strains of Francisella genus bacteria by quantitative iodometric method.
RESULTS: All the strains regardless of subspecies membership were characterized by high resistance to beta-lactam antibiotics, and bacteria of the mediasiatica subspecies in contrast to other francisella did not synthesize beta-lactamase. Our attempts to induce beta-lactamase activity in vitro and in vivo in strains of this subspecies did not succeed. A method of intraspecies differentiation of F. tularensis by nitorcefin disks is proposed based on the distinctive feature.
CONCLUSION: A high level of F. tularensis subsp. mediasiatica resistance to beta-lactams in vitro and their inefficiency during therapy of experimental tularemia due to a beta-lactamase negative strain suggests that F. tularensis beta-lactamase is not the leading factor in formation of native penicillin resistance of tularemia causative agent.