Arteriosclerosis, thrombosis, and vascular biology · 2013
Bone morphogenetic proteins protect pulmonary microvascular endothelial cells from apoptosis by upregulating α-B-crystallin.
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Ciumas Mariana, Eyries Mélanie, Poirier Odette, Maugenre Svetlana, Dierick France, Gambaryan Natalia, Montagne Kevin, Nadaud Sophie, Soubrier Florent
Аннотация
OBJECTIVE: To investigate the role of bone morphogenetic proteins (BMPs) on α-B-crystallin (CRYAB) expression and its physiological consequences on endothelial cells (ECs).
APPROACH AND RESULTS: We report that the gene encoding for the small heat shock protein, CRYAB, is a transcriptional target of the BMP signaling pathway. We demonstrate that CRYAB expression is upregulated strongly by BMPs in an EC line and in human lung microvascular ECs and human umbilical vein ECs. We show that BMP signals through the BMPR2-ALK1 pathway to upregulate CRYAB expression through a transcriptional indirect mechanism involving Id1. We observed that the known antiapoptotic effect of the BMPs is, in part, because of the upregulation of CRYAB expression in EC. We also show that cryab is downregulated in vivo, in a mouse model of pulmonary arterial hypertension induced by chronic hypoxia where the BMP pathway is downregulated.
CONCLUSIONS: We demonstrate a cross-talk between BMPs and CRYAB and a major effect of this regulatory interaction on resistance to apoptosis.
APPROACH AND RESULTS: We report that the gene encoding for the small heat shock protein, CRYAB, is a transcriptional target of the BMP signaling pathway. We demonstrate that CRYAB expression is upregulated strongly by BMPs in an EC line and in human lung microvascular ECs and human umbilical vein ECs. We show that BMP signals through the BMPR2-ALK1 pathway to upregulate CRYAB expression through a transcriptional indirect mechanism involving Id1. We observed that the known antiapoptotic effect of the BMPs is, in part, because of the upregulation of CRYAB expression in EC. We also show that cryab is downregulated in vivo, in a mouse model of pulmonary arterial hypertension induced by chronic hypoxia where the BMP pathway is downregulated.
CONCLUSIONS: We demonstrate a cross-talk between BMPs and CRYAB and a major effect of this regulatory interaction on resistance to apoptosis.
Предметные рубрики MeSH
Activin Receptors, Type IIAnimalsApoptosisBone Morphogenetic Protein 4Bone Morphogenetic Protein 7Bone Morphogenetic Protein Receptors, Type IIBone Morphogenetic ProteinsDisease Models, AnimalEndothelial CellsFamilial Primary Pulmonary HypertensionGrowth Differentiation Factor 2Growth Differentiation FactorsHuman Umbilical Vein Endothelial CellsHumansHypertension, PulmonaryLungMiceRNA, Small InterferingSignal TransductionUp-Regulationalpha-Crystallin B Chain