Effect of Sheng Xuexiaoban capsules on Tregs in an ITP mouse model.

OBJECTIVES

To study the effect of Sheng Xuexiaoban capsules (SXXCs) on restoring immune dysfunction in ITP model mice.

METHODS

We assessed changes in platelet counts, megakaryocyte counts, Treg functions, interleukin-2 expression, and other indicators to investigate the effect of SXXCs in the ITP mouse model.

RESULTS

After treatment of the ITP mouse model with SXXCs, we found increases in platelets (577.35 ± 76.94 vs. 123.33 ± 16.24, P < 0.05) and the Treg number (7.35 ± 1.94 vs. 1.33 ± 1.24), and Foxp3 mRNA expression (2-ΔCt: 0.2493 ± 0.03102 vs. 0.683 ± 0.05774, P = 0.0027). Decreased interleukin-2 produced by effector T cells was detected (2-ΔCt: 0.6119 ± 0.01173 vs. 2.838 ± 0.1004, P < 0.001). SXXCs treatment significantly improved the proportion and function of Tregs. By increasing Foxp3 mRNA expression and decreasing interleukin-2, we restored immune tolerance to achieve a good therapeutic effect in ITP model mice.

CONCLUSIONS

We confirmed that SXXCs have a good therapeutic effect on ITP. Simultaneously we verified the important role of Tregs in ITP pathogenesis.