Ophthalmology · 1999
Choroidal tuberculosis diagnosed by polymerase chain reaction. A clinicopathologic case report.
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Bowyer J D, Gormley P D, Seth R, Downes R N, Lowe J
Аннотация
PURPOSE: To show the use of the polymerase chain reaction (PCR) in a granulomatous choroidal lesion to support a diagnosis of tuberculosis.
DESIGN: Observational case report.
TESTING: Nucleic acid target amplification of a choroidal specimen using PCR for detection of Mycobacterium tuberculosis was tested.
MAIN OUTCOME MEASURES: Positive nucleic acid target amplification for M. tuberculosis in the ocular sample was measured.
RESULTS: PCR was positive for M. tuberculosis with appropriate negative controls.
CONCLUSIONS: PCR was thought to be a useful supportive technique in the diagnosis of choroidal tuberculosis.
DESIGN: Observational case report.
TESTING: Nucleic acid target amplification of a choroidal specimen using PCR for detection of Mycobacterium tuberculosis was tested.
MAIN OUTCOME MEASURES: Positive nucleic acid target amplification for M. tuberculosis in the ocular sample was measured.
RESULTS: PCR was positive for M. tuberculosis with appropriate negative controls.
CONCLUSIONS: PCR was thought to be a useful supportive technique in the diagnosis of choroidal tuberculosis.