Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2005
[Lactase and its gene regulation in BALB/c mice at different ages].
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He Wei, Lü Bin, Huang Chengyu
Аннотация
OBJECTIVE: In order to clarify the trend of change in lactase and its gene regulation pattern, the authors determined the lactase activities and mRNA levels in BALB/c mice at different ages.
METHODS: The lactase activities and mRNA levels of the 3-, 5-, 7-, 9- week old mice were determined by the modified method of Dahlqvist and in situ hybridization respectively.
RESULTS: The 3 wk old mice had the highest lactase activities, to the contrary, the 9 wk old mice had the lowest lactase activities. Meanwhile, the lactase mRNA levels displayed no diversities.
CONCLUSION: The mice had higher lactase activities before weaning, but significantly very low activities after weaning. These findings indicate that BALB/c mice have the same adult-type hypolactasia as human. No statistically significant difference in lactase mRNA levels between the 4 age-groups implies that lactase gene is regulated at the translational and/or post-translational levels, but not at the transcriptional level.
METHODS: The lactase activities and mRNA levels of the 3-, 5-, 7-, 9- week old mice were determined by the modified method of Dahlqvist and in situ hybridization respectively.
RESULTS: The 3 wk old mice had the highest lactase activities, to the contrary, the 9 wk old mice had the lowest lactase activities. Meanwhile, the lactase mRNA levels displayed no diversities.
CONCLUSION: The mice had higher lactase activities before weaning, but significantly very low activities after weaning. These findings indicate that BALB/c mice have the same adult-type hypolactasia as human. No statistically significant difference in lactase mRNA levels between the 4 age-groups implies that lactase gene is regulated at the translational and/or post-translational levels, but not at the transcriptional level.