Journal of gastroenterology and hepatology · 2009
Protecting effects of a large dose of dexamethasone on spleen injury of rats with severe acute pancreatitis.
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Xiping Zhang, Ruiping Zhang, Binyan Yu, Li Zhou, Hanqing Chen, Wei Zhu, Rongchao Ying, Jing Ye, Wenqin Yuan, Jinjin Bai
Аннотация
BACKGROUND AND AIMS: To explore the protecting effects and mechanisms of dexamethasone on spleen injury in rats with severe acute pancreatitis (SAP).
METHODS: The rats were randomly divided into a model control group, treated group and sham-operated group. The contents of plasma endotoxin, serum NO, phospholipase A(2) enzyme (PLA(2)) and endothelin-1 (ET-1) were determined. The mortality rate, pathological changes and changes of Bax and Bcl-2 protein expression levels and apoptotic indexes in the spleen of rats were observed in all groups, respectively, at 3, 6 and 12 h after operation.
RESULTS: Although the survival rate was significantly higher in the treated group than in the model control group, there was no significantly different between them (P > 0.05). The expression levels of Bax and Bcl-2 proteins and apoptotic indexes were significantly higher in the treated group than in the model control group at different time points (P < 0.05 or P < 0.01) while other blood indexes contents and pathological severity scores of spleen were significantly lower in the treated group than in the model control group (P < 0.05, P < 0.01 or P < 0.001).
CONCLUSION: Dexamethasone can protect spleen from injury during SAP mainly by reducing the content of inflammatory mediators in blood.
METHODS: The rats were randomly divided into a model control group, treated group and sham-operated group. The contents of plasma endotoxin, serum NO, phospholipase A(2) enzyme (PLA(2)) and endothelin-1 (ET-1) were determined. The mortality rate, pathological changes and changes of Bax and Bcl-2 protein expression levels and apoptotic indexes in the spleen of rats were observed in all groups, respectively, at 3, 6 and 12 h after operation.
RESULTS: Although the survival rate was significantly higher in the treated group than in the model control group, there was no significantly different between them (P > 0.05). The expression levels of Bax and Bcl-2 proteins and apoptotic indexes were significantly higher in the treated group than in the model control group at different time points (P < 0.05 or P < 0.01) while other blood indexes contents and pathological severity scores of spleen were significantly lower in the treated group than in the model control group (P < 0.05, P < 0.01 or P < 0.001).
CONCLUSION: Dexamethasone can protect spleen from injury during SAP mainly by reducing the content of inflammatory mediators in blood.
Предметные рубрики MeSH
Acute DiseaseAnimalsAnti-Inflammatory AgentsApoptosisDexamethasoneDisease Models, AnimalEndothelin-1EndotoxinsInflammation MediatorsMaleNitric OxidePancreatitisPhospholipases A2Proto-Oncogene Proteins c-bcl-2RatsRats, Sprague-DawleySeverity of Illness IndexSpleenSplenic DiseasesTaurocholic AcidTime Factorsbcl-2-Associated X Protein