Future microbiology · 2014
Immunoreactivity of synthetic peptides derived from proteins of Cryptococcus gattii.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
de Serpa Brandão Rafael Melo Santos, Soares Martins Liline Maria, de Andrade Hélida Monteiro, Faria Angélica Rosa, Soares Leal Maria José, da Silva Adalberto Socorro, Wanke Bodo, dos Santos Lazéra Márcia, Vainstein Marilene Henning, Mendes Rinaldo Poncio, Moris Daniela Vanessa, de Souza Cavalcante Ricardo, do Monte Semiramis Jamil Hadad
Аннотация
AIM: To determine the immunoreactivity of synthetic Cryptococcus-derived peptides.
MATERIALS & METHODS: A total of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISAs. The peptides were first evaluated for their ability to react against sera from immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high sensitivity and specificity in the first test were then retested with sera from individuals with other fungal pathologies for cross-reactivity determination.
RESULTS: Six of 63 synthetic peptides were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity.
CONCLUSION: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins.
MATERIALS & METHODS: A total of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISAs. The peptides were first evaluated for their ability to react against sera from immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high sensitivity and specificity in the first test were then retested with sera from individuals with other fungal pathologies for cross-reactivity determination.
RESULTS: Six of 63 synthetic peptides were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity.
CONCLUSION: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins.