Archives of medical research · 2012
Expression of beta defensin 2 in experimental pulmonary tuberculosis: tentative approach for vaccine development.
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Rivas-Santiago Bruno, Cervantes-Villagrana Alberto, Sada Eduardo, Hernández-Pando Rogelio
Аннотация
BACKGROUND AND AIMS: Defensins are low molecular weight antimicrobial and immunomodulatory peptides. Their participation against Mycobacterium tuberculosis (MTb) infection has been scarcely studied.
METHODS: We describe the kinetics of murine β-defensin 2 (mBD-2) expression by quantitative real-time PCR and cellular location by immunohistochemistry in murine models of progressive pulmonary tuberculosis and latent infection.
RESULTS: During progressive disease, mBD2 gene expression raised its peak at 14 days postinfection, whereas in latent infection it was at 90 days. In both models, mBD-2 immunostaining was essentially located in cells with dendritic morphology located near mediastinal lymph nodes, which correlated with the previous reported highest expression of cell-mediated protected immunity in both models.
CONCLUSIONS: These results suggest that mBD-2 may play a role in the control of bacilli growth by contributing to establish a Th1 response, being a link between innate and adaptative immunity. These data may be used for the development of new vaccine approaches.
METHODS: We describe the kinetics of murine β-defensin 2 (mBD-2) expression by quantitative real-time PCR and cellular location by immunohistochemistry in murine models of progressive pulmonary tuberculosis and latent infection.
RESULTS: During progressive disease, mBD2 gene expression raised its peak at 14 days postinfection, whereas in latent infection it was at 90 days. In both models, mBD-2 immunostaining was essentially located in cells with dendritic morphology located near mediastinal lymph nodes, which correlated with the previous reported highest expression of cell-mediated protected immunity in both models.
CONCLUSIONS: These results suggest that mBD-2 may play a role in the control of bacilli growth by contributing to establish a Th1 response, being a link between innate and adaptative immunity. These data may be used for the development of new vaccine approaches.
Предметные рубрики MeSH
Adaptive ImmunityAnimalsCrosses, GeneticDendritic CellsDisease ProgressionGene Expression RegulationImmunity, CellularImmunoenzyme TechniquesLatent TuberculosisLungLymphatic VesselsMaleMediastinumMiceMice, Inbred BALB CMice, Inbred DBAReal-Time Polymerase Chain ReactionSpecific Pathogen-Free OrganismsTh1 CellsTuberculosis VaccinesTuberculosis, Pulmonarybeta-Defensins