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Войти в исследования Parasites & vectors · 2025
Molecular characterization of potential Plasmodium-Blocking Serratia spp. bacteria in field-caught malaria mosquito in Burkina Faso.
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Traoré Haoua, Gnambani Edounou Jacques, Hien Domonbabele François de Sales, Yao Raymond Karlhis, Konkobo Maurice, Sodré Aicha Fatimata, Somda Martin Bienvenu, Ouedraogo Abdoul Salam, Diabaté Abdoulaye, Bilgo Etienne
Аннотация
BACKGROUND: One of the alternatives for controlling malaria is using mosquito symbiotic bacteria to reduce Plasmodium transmission. Species of Serratia, a genus of the Enterobacteriaceae family, have been isolated from the midgut of mosquitoes and are commonly found in water, soil and plant surfaces. These bacteria have shown great promise in blocking the transmission of Plasmodium in mosquitoes. The aim of this study was to isolate and characterize the genus Serratia within the Anopheles gambiae complex from Burkina Faso.
METHODS: Mosquitoes were collected in three field sites located in Houet Province in western Burkina Faso (Dioulassoba, Vallée du Kou and Soumousso), transported to the laboratory and identified morphologically. The salivary gland, midgut, spermatheca, ovary of females and testis of males were dissected and their contents ground up. Different species of Serratia were identified by PCR targeting of the luxS gene of Serratia, followed by 16S ribosomal RNA (rRNA) sequencing.
RESULTS: Molecular analyses identified the isolates as belonging to the genus Serratia, and phylogenetic reconstruction revealed that these strains are highly similar to one another but distinct from Serratia strains previously reported in neighboring countries such as Ghana and Nigeria. The overall prevalence of Serratia among malaria vectors was 13.3%. This prevalence varied according to the development stage of mosquitoes, locality of origin and mosquito organ. Only one Anopheles coluzzii mosquito was co-infected with Serratia and Plasmodium falciparum.
CONCLUSIONS: The results of this study support the presence of Serratia spp. in wild mosquitoes from Burkina Faso, we well as their potential use in malaria control.
METHODS: Mosquitoes were collected in three field sites located in Houet Province in western Burkina Faso (Dioulassoba, Vallée du Kou and Soumousso), transported to the laboratory and identified morphologically. The salivary gland, midgut, spermatheca, ovary of females and testis of males were dissected and their contents ground up. Different species of Serratia were identified by PCR targeting of the luxS gene of Serratia, followed by 16S ribosomal RNA (rRNA) sequencing.
RESULTS: Molecular analyses identified the isolates as belonging to the genus Serratia, and phylogenetic reconstruction revealed that these strains are highly similar to one another but distinct from Serratia strains previously reported in neighboring countries such as Ghana and Nigeria. The overall prevalence of Serratia among malaria vectors was 13.3%. This prevalence varied according to the development stage of mosquitoes, locality of origin and mosquito organ. Only one Anopheles coluzzii mosquito was co-infected with Serratia and Plasmodium falciparum.
CONCLUSIONS: The results of this study support the presence of Serratia spp. in wild mosquitoes from Burkina Faso, we well as their potential use in malaria control.