Annals of clinical and translational neurology · 2019
Identification of serum microRNAs as potential biomarkers in Pompe disease.
Клиническое исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Carrasco-Rozas Ana, Fernández-Simón Esther, Lleixà Maria Cinta, Belmonte Izaskun, Pedrosa-Hernandez Irene, Montiel-Morillo Elena, Nuñez-Peralta Claudia, Llauger Rossello Jaume, Segovia Sonia, De Luna Noemí, Suarez-Calvet Xavier, Illa Isabel, Pompe Spanish Study group, Díaz-Manera Jordi, Gallardo Eduard
Аннотация
OBJECTIVE: To analyze the microRNA profile in serum of patients with Adult Onset Pompe disease (AOPD).
METHODS: We analyzed the expression of 185 microRNAs in serum of 15 AOPD patients and five controls using microRNA PCR Panels. The expression levels of microRNAs that were deregulated were further studied in 35 AOPD patients and 10 controls using Real-Time PCR. Additionally, the skeletal muscle expression of microRNAs which showed significant increase levels in serum samples was also studied. Correlations between microRNA serum levels and muscle function test, spirometry, and quantitative muscle MRI were performed (these data correspond to the study NCT01914536 at ClinicalTrials.gov).
RESULTS: We identified 14 microRNAs that showed different expression levels in serum samples of AOPD patients compared to controls. We validated these results in a larger cohort of patients and we found increased levels of three microRNAs, the so called dystromirs: miR-1-3p, miR-133a-3p, and miR-206. These microRNAs are involved in muscle regeneration and the expression of these was increased in patients' muscle biopsies. Significant correlations between microRNA levels and muscle function test were found.
INTERPRETATION: Serum expression levels of dystromirs may represent additional biomarkers for the follow-up of AOPD patients.
METHODS: We analyzed the expression of 185 microRNAs in serum of 15 AOPD patients and five controls using microRNA PCR Panels. The expression levels of microRNAs that were deregulated were further studied in 35 AOPD patients and 10 controls using Real-Time PCR. Additionally, the skeletal muscle expression of microRNAs which showed significant increase levels in serum samples was also studied. Correlations between microRNA serum levels and muscle function test, spirometry, and quantitative muscle MRI were performed (these data correspond to the study NCT01914536 at ClinicalTrials.gov).
RESULTS: We identified 14 microRNAs that showed different expression levels in serum samples of AOPD patients compared to controls. We validated these results in a larger cohort of patients and we found increased levels of three microRNAs, the so called dystromirs: miR-1-3p, miR-133a-3p, and miR-206. These microRNAs are involved in muscle regeneration and the expression of these was increased in patients' muscle biopsies. Significant correlations between microRNA levels and muscle function test were found.
INTERPRETATION: Serum expression levels of dystromirs may represent additional biomarkers for the follow-up of AOPD patients.