[Relationship and significance among IL-6,PI3K/Akt and GSK 3β in chronic rhinosinusitis].

Objective

To explore the relationship and significance among the expressions of cytokines IL-6，PI3K/Akt signaling transduction pathway, and GSK3β in chronic rhinosinusitis.Method:The proteins for IL-6,PI3K,Akt,GSK3β were assayed by Western blot, and mRNAs of cytokines IL-6,IL-6 receptor were measured by real-time quantitative polymerase chain reaction(qPCR) in nasal tissue from the patients with chronic rhinosinusitis with nasal polyps(CRSwNP),chronic rhinosinusitis without nasal polyps(CRSsNP) and control subjects. Result:IL-6,PI3K,Akt,GSK3β proteins in CRS were higher than those in the control subjects, the differences were statistically significant(P<0.05). There was no significant difference between the groups of CRSsNP and CRSwNP. There were significant differences of cytokines IL-6 mRNA expression between CRSsNP, CRSwNP and control group(P<0.05).IL-6 exists in two forms glycosylated(55 kd) and non-glycosylated(25 kd) in CRS. CRSsNP group mainly glycosylated (glycosylated and non-glycosylated protein expression ratio is about 2.4：1)，CRSwNP group mainly non-glycosylated(glycosylated and non-glycosylated protein expression ratio values about 0.4：1).There was a positive correlation trend among the expressions of IL-6,PI3K,Akt and GSK3β in CRS.

Conclusion

The abnormal expressions of IL-6,PI3K,Akt and GSK3β in the nasal mucosa of CRS may play a pro-inflammatory role in the occurrence and development of CRS. The glycosylated IL-6 and the non-glycosylated IL-6 may be both involved in the inflammation of CRS.