Morphological evaluation of the effectiveness of probiotic disinfectants in the treatment of experimental widespread peritonitis.

OBJECTIVE

Aim: to conduct a morphological evaluation of the effectiveness of probiotic disinfectants for abdominal cavity sanitation in experimental widespread peritonitis.

PATIENTS AND

METHODS

Materials and

Methods

The experimental study was conducted on 45 white male Wistar rats aged 4-5 months, which were modelled with peritonitis. The rats were divided into 3 groups of 15 animals each. The treatment of animals in the main group (group I) consisted of washing the abdominal cavity with a 5% solution of probiotic disinfectant, applying a probiotic disinfectant spray and a gel with probiotics to the surgical wound. For the treatment of rats in the comparison group (group II), a sorbent solution was used. The treatment of rats in the placebo control group (group III) consisted of abdominal lavage with 0.9% NaCl. Histological, histochemical and immunohistochemical studies were performed.

RESULTS

Results

In the main group, 10 (66.7 %) rats survived, in which peritonitis was eliminated, as evidenced by clinical and morphological results. In the comparison group, 8 (53.3 %) rats survived, in which peritonitis was eliminated. All animals in the placebo control group died of severe peritonitis. The best repair and renewal of mesothelial cells with a pronounced intensity of parietal peritoneal expression was in group I. In group II on the 7th day there were mesothelial cells with moderate expression, in group III - there was a weak intensity of expression in single cells.

CONCLUSION

Conclusions

According to our data, probiotic disinfectants are effective for abdominal cavity sanitation in peritonitis, which was proved by experimental study.