The effect of ethanol upon early development in mice and rats. XX. The effect of chronic biparental beer intake in mice upon early implantation events.

The effect of chronic biparental beer intake upon early implantation in mice was investigated. In order to study the changes taking place in the blastocyst and locally in the uterus at the site of blastocyst attachment, the uteri were tested for the presence of Evans blue areas (Psychoyos test), histochemically for alkaline phosphatase in the stroma and histologically examined. It resulted that the beverage used has a noxious effect upon the implantation process, manifested by: delayed attachment of blastocysts, decreased intensity or absence of decidual reaction, the presence of dead cells in the decidua, the desynchronisation of the implantation process and the diminution or absence of alkaline phosphatase reaction. These results may explain some of our previous observations regarding the late (fetal) effect of preconceptional intake of beer.