Acta ophthalmologica · 2025
Epidermal growth factor receptor antibody and axial elongation in experimental myopia.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Dong Li, Zhou Wen-Da, Yang Yu-Hang, Zhang Ru-Heng, Zhao Han-Qing, Yu Chu-Yao, Li He-Yan, Wang Shan-Shan, Wu Hao-Tian, Dong Jiao-Yue, Shi Xu-Han, Zheng Ze-Tong, Jonas Jost B, Wei Wen-Bin
Аннотация
PURPOSE: To evaluate the effect of intraocularly applied epidermal growth factor receptor (EGFR) antibody panitumumab on axial elongation.
METHODS: This is a preclinical, safety, and efficacy experimental study. Guinea pigs aged 2-3 weeks were fitted with bilateral plano lenses (Group I; n = 10) or underwent bilateral lens-induced myopization (LIM) (Group II; n = 10) with no intravitreal injections. Animals with LIM of Groups III (n = 15), IV (n = 15) and V (n = 15) received three 4-weekly intravitreal injections of panitumumab at doses of 25, 50 and 100 μg, respectively, into their right eyes, and of phosphate-buffered saline into their left eyes.
RESULTS: Inter-eye differences in axial elongation (right eye minus left eye at study end) decreased (p < 0.001) from 0.00 ± 0.02 mm (Group I) and - 0.01 ± 0.02 mm (Group II) to -0.09 ± 0.03 mm (Group III), -0.12 ± 0.03 mm (Group IV) and - 0.17 ± 0.05 mm (Group V). Interocular choroidal thickness differences (measured by optical coherence tomography) increased (p < 0.01) from Groups I and II to Groups III-V. Western blot analysis showed an increase (p < 0.05) in expression levels of EGFR and its downstream phosphorylated signalling molecule, p-PI3K, in Group II compared to Group I and a decrease in Groups III-V compared to Group II (all p < 0.01). Interocular differences in retinal thickness did not differ significantly (p > 0.05) between the groups. TUNEL staining revealed no significant differences in retinal apoptotic cell density among any groups (all p > 0.05).
CONCLUSIONS: Intravitreally administered panitumumab in young guinea pigs with LIM resulted in a dose-dependent and treatment frequency-dependent reduction in axial elongation, supporting the role of EGFR signalling in axial elongation.
METHODS: This is a preclinical, safety, and efficacy experimental study. Guinea pigs aged 2-3 weeks were fitted with bilateral plano lenses (Group I; n = 10) or underwent bilateral lens-induced myopization (LIM) (Group II; n = 10) with no intravitreal injections. Animals with LIM of Groups III (n = 15), IV (n = 15) and V (n = 15) received three 4-weekly intravitreal injections of panitumumab at doses of 25, 50 and 100 μg, respectively, into their right eyes, and of phosphate-buffered saline into their left eyes.
RESULTS: Inter-eye differences in axial elongation (right eye minus left eye at study end) decreased (p < 0.001) from 0.00 ± 0.02 mm (Group I) and - 0.01 ± 0.02 mm (Group II) to -0.09 ± 0.03 mm (Group III), -0.12 ± 0.03 mm (Group IV) and - 0.17 ± 0.05 mm (Group V). Interocular choroidal thickness differences (measured by optical coherence tomography) increased (p < 0.01) from Groups I and II to Groups III-V. Western blot analysis showed an increase (p < 0.05) in expression levels of EGFR and its downstream phosphorylated signalling molecule, p-PI3K, in Group II compared to Group I and a decrease in Groups III-V compared to Group II (all p < 0.01). Interocular differences in retinal thickness did not differ significantly (p > 0.05) between the groups. TUNEL staining revealed no significant differences in retinal apoptotic cell density among any groups (all p > 0.05).
CONCLUSIONS: Intravitreally administered panitumumab in young guinea pigs with LIM resulted in a dose-dependent and treatment frequency-dependent reduction in axial elongation, supporting the role of EGFR signalling in axial elongation.