Oral oncology · 2014
Development and characterization of salivary adenoid cystic carcinoma cell line.
Клинический случайСтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
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Ошибки и ограничения
История · было → стало
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Li Jie, Perlaky Laszlo, Rao Pulivarthi, Weber Randal S, El-Naggar Adel K
Аннотация
OBJECTIVE: To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.
MATERIALS AND METHODS: Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.
RESULTS: Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.
CONCLUSION: We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.
MATERIALS AND METHODS: Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.
RESULTS: Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.
CONCLUSION: We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.