Development and characterization of salivary adenoid cystic carcinoma cell line.

OBJECTIVE

To develop in vitro adenoid cystic carcinoma cell line as a surrogate for functional studies.

MATERIALS AND

METHODS

Cells obtained from a primary ACC of the base of tongue were cultivated in vitro and immortalized with h-TERT. Morphologic, cytogenetic and functional studies were performed.

RESULTS

Tumor cells were verified by positive reactions to keratin and smooth muscle actin and phenotypic cellular and nuclear features. In-vitro cell growth and colony formation assay supported their tumor nature.

CONCLUSION

We authenticated an ACC cell line with hybrid epithelial-myoepithelial feature as a resource for functional experimentation.