Rapid diagnosis of sporotrichosis by immunofluorescent method.

Histopathological examinations fungal cultures and direct immunofluorescent staining of the pus and skin biopsy microsections with fluorescein isothiocyanate-labelled specific antiserum were performed for 13 patients suspected to be infected with Sporotrichum schenckii. While histopathological examinations could not give useful information, the latter two gave more reliable results which could support the clinical diagnosis. The direct immunofluorescent staining test could be used as a method for rapid diagnosis of sporotrichosis.