Microbial pathogenesis · 2026
A novel circular RNA circPRIM2 revealed by circular RNA profile inhibits mammary epithelial cell proliferation and immune response in bovine mastitis.
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Yang Wei, Guo Jia-Tong, Gao Jing, Niu Yichen, Dong Xinnan, Luo Cheng-Hua, Xie Xiaogang, Li Dan, Gao Ming-Qing
Аннотация
BACKGROUND: Bovine mastitis is a major inflammatory disease that impairs mammary epithelial function, milk production, and animal health. Circular RNAs (circRNAs) are involved in many physiological and pathological processes, but their roles in pathogen-induced bovine mammary epithelial dysfunction remain unclear.
AIM: This study aimed to identify mastitis-associated circRNAs and determine whether circPRIM2 regulates mammary epithelial cell proliferation, β-casein secretion, and inflammatory responses.
METHODS: An inflammatory model was established in bovine mammary alveolar cell-T (MAC-T) cells by heat-inactivated Staphylococcus aureus or Escherichia coli stimulation. circRNA profiles were analyzed by high-throughput sequencing and bioinformatic prediction. The stability and function of circPRIM2 were further validated by RNase R digestion, RT-qPCR, CCK-8, EdU, Western blot, and ELISA assays.
RESULTS: A total of 5043 circRNAs were identified in S. aureus-treated MAC-T cells, and 31 circRNAs were differentially expressed between S. aureus-treated and control cells. circPRIM2, a novel circRNA derived from the PRIM2 locus, was down-regulated under inflammatory conditions and resistant to RNase R digestion. circPRIM2 overexpression reduced MAC-T cell viability and EdU incorporation, increased β-casein expression and secretion, and decreased LTA-induced TNF-α and IL-1β secretion as well as p65 phosphorylation.
CONCLUSION: These findings suggest that circPRIM2 may act as an epithelial regulatory circRNA linking cell growth, secretory function, and immune response during bovine mastitis. This study provides a basis for further exploring circPRIM2 as a potential biomarker or molecular target for mastitis management.
AIM: This study aimed to identify mastitis-associated circRNAs and determine whether circPRIM2 regulates mammary epithelial cell proliferation, β-casein secretion, and inflammatory responses.
METHODS: An inflammatory model was established in bovine mammary alveolar cell-T (MAC-T) cells by heat-inactivated Staphylococcus aureus or Escherichia coli stimulation. circRNA profiles were analyzed by high-throughput sequencing and bioinformatic prediction. The stability and function of circPRIM2 were further validated by RNase R digestion, RT-qPCR, CCK-8, EdU, Western blot, and ELISA assays.
RESULTS: A total of 5043 circRNAs were identified in S. aureus-treated MAC-T cells, and 31 circRNAs were differentially expressed between S. aureus-treated and control cells. circPRIM2, a novel circRNA derived from the PRIM2 locus, was down-regulated under inflammatory conditions and resistant to RNase R digestion. circPRIM2 overexpression reduced MAC-T cell viability and EdU incorporation, increased β-casein expression and secretion, and decreased LTA-induced TNF-α and IL-1β secretion as well as p65 phosphorylation.
CONCLUSION: These findings suggest that circPRIM2 may act as an epithelial regulatory circRNA linking cell growth, secretory function, and immune response during bovine mastitis. This study provides a basis for further exploring circPRIM2 as a potential biomarker or molecular target for mastitis management.