Journal of medical primatology · 2025
Investigation of Anti-Toxoplasma gondii Antibodies and Molecular Analysis in Nonhuman Primates Under Human Care in Rio de Janeiro, Brazil.
СтатьяНазвание и аннотация: Перевод в очереди
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Zorzi Natalie Renata, Segalin Júlia Trevizan, Dazzi Cláudia, Oliveira Franciele, da Silva Andressa Ferreira, Alves Leila Maria Carvalho, Athayde-Gusmão Anna Elisa, Gianfrancisco Olivia Zen, Balthazar Daniel Almeida, Rocha Fernanda Correa, Lopes Clarissa Vieira, Baêta Bruna Azevedo, Cordeiro Matheus Dias, Moreira Silvia Bahadian, Pissinatti Alcides, Ubiali Daniel Guimarães, Costa Márcio Machado, Vieira Maria Isabel Botelho
Аннотация
INTRODUCTION: This study aimed to conduct a serological and molecular survey of Toxoplasma gondii in nonhuman primates under human care in the state of Rio de Janeiro.
MATERIALS AND METHODS: MAT and qPCR were performed on 199 serum and blood samples collected from animals at the following locations: BioParque Zoo, Volta Redonda Municipal Zoo, Wildlife Rehabilitation Center, Laboratory Animal Facility of the Federal University of Rio de Janeiro, and the Rio de Janeiro Primatology Center. Risk factors for T. gondii infection were also assessed.
RESULTS: qPCR analysis revealed DNA amplification of T. gondii in one animal of the species Alouatta caraya. MAT analysis showed 100% seropositivity among Old World primates and 42.62% among New World primates.
CONCLUSION: At locations where raw meat is included in the diet and water is unfiltered, animals were found to be 10.88 times more likely to test seropositive.
MATERIALS AND METHODS: MAT and qPCR were performed on 199 serum and blood samples collected from animals at the following locations: BioParque Zoo, Volta Redonda Municipal Zoo, Wildlife Rehabilitation Center, Laboratory Animal Facility of the Federal University of Rio de Janeiro, and the Rio de Janeiro Primatology Center. Risk factors for T. gondii infection were also assessed.
RESULTS: qPCR analysis revealed DNA amplification of T. gondii in one animal of the species Alouatta caraya. MAT analysis showed 100% seropositivity among Old World primates and 42.62% among New World primates.
CONCLUSION: At locations where raw meat is included in the diet and water is unfiltered, animals were found to be 10.88 times more likely to test seropositive.