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DOI 10.1007/s00417-006-0503-3

Медицинская библиотека · Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2006

Pulsed-field gel electrophoresis in the identification of the origin of bacterial keratitis caused by Pseudomonas aeruginosa.

de Melo Gustavo Barreto, Aggio Fabio Bom, Höfling-Lima Ana Luisa, d'Azevedo Pedro Alves, Pignatari Antônio Carlos Campos

Клинический случайСтатьяПеревод пока не подготовлен

Аннотация

PURPOSE: To report the use of pulsed-field gel electrophoresis (PFGE) in the investigation of the origin of infectious keratitis.

METHODS

A 19-year-old girl presented with infectious keratitis in the left eye. She was a soft contact lens wearer, and was noncompliant with the proper cleaning methods proposed by the manufacturer. Microbiological assessment was performed by means of culture and PFGE.

RESULTS

The patient was treated successfully with topical cefalotin and gentamicin drops. Cultures were positive for Pseudomonas aeruginosa in the cornea and conjunctiva, and in the contact lens, its case and the cleaning solution. PFGE showed identical patterns of banding in each.

CONCLUSION

In this case of bacterial keratitis, PFGE proved to be very useful in identifying how the contamination occurred.

Для цитирования: de Melo Gustavo Barreto, Aggio Fabio Bom, Höfling-Lima Ana Luisa, d'Azevedo Pedro Alves, Pignatari Antônio Carlos Campos. Pulsed-field gel electrophoresis in the identification of the origin of bacterial keratitis caused by Pseudomonas aeruginosa.. Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. 2006. DOI: 10.1007/s00417-006-0503-3.

Предметные рубрики MeSH

AdultAnti-Bacterial AgentsCephalothinConjunctivaContact LensesCorneaCorneal UlcerDNA, BacterialDrug Therapy, CombinationElectrophoresis, Gel, Pulsed-FieldEye Infections, BacterialFemaleGentamicinsHumansPseudomonas InfectionsPseudomonas aeruginosa
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