Guiding Principles: Reporting Elements for Gastrointestinal Organoid Research.

Gastrointestinal (GI) organoids are now widely used for disease modeling, drug discovery, regenerative-, and precision medicine. As publications using GI organoids have increased exponentially, methodological reporting across GI organoid studies remains inconsistent, making results difficult to interpret, compare, and reproduce. To address this, we present community-informed guidance for reporting key experimental details in GI organoid research. Our recommendations were developed by integrating established biomedical reporting frameworks, organoid-focused resources from international organizations, and structured input from the GI organoid community through an expert panel survey. These inputs informed a core set of reporting items, including stem cell source and donor context, media and supplement composition, extracellular matrix type, culture configuration, and other parameters essential for replication. We provide an "Organoid Reporting Toolkit" containing practical checklists and templates intended to help authors, reviewers, editors, and journals. Together, these resources aim to improve transparency and reproducibility, facilitate cross-study comparison, and streamline communication in the GI organoid field while preserving flexibility for methodological innovation.