Preparation, Characteristics, Antioxidant and Anti-Inflammatory Activity of SeNPs and ZnO-enriched Limosilactobacillus reuteri LR1.

Trace elements are crucial for animal development, but current additives like sodium selenite (Na2SeO3) and zinc sulfate (ZnSO4) pose toxicity and low bioavailability risks. Additionally, finding alternatives to antibiotics is a pressing concern. This study used a safe and eco-friendly method to create SeNPs and ZnO-enriched Limosilactobacillus reuteri LR1 (L. reuteri LR1-SeNPs-ZnO). And its characteristics, antibacterial and antioxidant activities were studied. These results showed that Na2SeO3 and ZnSO4 were transformed into SeNPs and ZnO by L. reuteri LR1, respectively. And it had significant bacteriostatic and antioxidant activities. Moreover, dietary supplementation of L. reuteri LR1-SeNPs-ZnO significantly increased the antioxidant and anti-inflammatory activities, and increased the expression level of tight junction protein in jejunum, and significantly improved intestinal microbiota dysbiosis induced by LPS. These results indicated that SeNPs and ZnO-enriched L. reuteri LR1 can replace antibiotics in maintaining intestinal health, with demonstrated benefits in enhancing antioxidant capacity and regulating inflammatory responses.