[Collagen synthesis and expression of connective tissue growth factor in the cultured fibroblasts of human hypertrophic scar].

OBJECTIVE

To explore the effect of connective tissue growth factor on the pathogenesis of human hypertrophic scar.

METHODS

Normal skin and hypertrophic scar fibroblasts were cultured in vitro. The collagen synthesis of fibroblasts were measured by H3-proline incorporation method. The expression of connective tissue growth factor protein and mRNA of fibroblasts were detected with immunocytochemistry staining and reverse transcription polymerase chain reaction methods.

RESULTS

Compared with normal skin fibroblast, the collagen synthesis and the expression of connective tissue growth factor protein and mRNA in the hypertrophic scar fibroblast was higher (P < 0.01).

CONCLUSION

Connective tissue growth factor may play an important role in promoting the fibrotic process of hypertrophic scar.