Zhonghua zheng xing shao shang wai ke za zhi = Zhonghua zheng xing shao shang waikf [i.e. waike] zazhi = Chinese journal of plastic surgery and burns · 1999
[Study on the expression of calreticulin in hypertrophic scar-derived fibroblasts].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Zhao Y, He Q
Аннотация
OBJECTIVE: To investigate the expression level and intracellular distribution of calreticulin(CRT) in hypertrophic scar-derived fibroblasts(HSF) and normal skin-derived fibroblasts(NSF).
METHODS: Using anti-CRT antibody, CRT was detected in HSF and NSF by immunocytochemistry and ELISA technique.
RESULTS: CRT was present in the cytoplasm and nuclei in proliferating HSF or NSF in culture, but HSF possessed significantly higher expression level of CRT than NSF(P < 0.01). When these cells (HSF and NSF) were fused, neither intracellular nor intranuclear staining for CRT was observed.
CONCLUSION: In HSF and NSF, the distribution of CRT is widespread. Because of its abundant expression in HSF, we postulate that CRT as a multifunctional protein plays an important role in Ca2+ sequestering, integrin-mediated signalling and cell adhesion.
METHODS: Using anti-CRT antibody, CRT was detected in HSF and NSF by immunocytochemistry and ELISA technique.
RESULTS: CRT was present in the cytoplasm and nuclei in proliferating HSF or NSF in culture, but HSF possessed significantly higher expression level of CRT than NSF(P < 0.01). When these cells (HSF and NSF) were fused, neither intracellular nor intranuclear staining for CRT was observed.
CONCLUSION: In HSF and NSF, the distribution of CRT is widespread. Because of its abundant expression in HSF, we postulate that CRT as a multifunctional protein plays an important role in Ca2+ sequestering, integrin-mediated signalling and cell adhesion.