Journal of ethnopharmacology · 2026
Anti-metastatic effect of Paris polyphylla var. yunnanensis and Paris fargesii in esophageal cancer preclinical models.
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Xie Jing, Yue Grace Gar-Lee, Ni Wei, Yu Ling-Ling, Gao Si, Ling Shan-Shan, Fang Xin, Bik-San Lau Clara, Liu Hai-Yang
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Rhizoma Paridis refers to the dried rhizomes of perennial herbaceous plants belonging to the genus Paris. Classified under the Melanthiaceae family, this medicinal herb has long been used in folk medicine in East Asia and Europe for the treatment of bleeding, diarrhea, gastritis, mumps, abscesses, as well as snake and insect bites.
AIM OF THE STUDY: This study aims to explore the anti-metastatic effects of the active fractions and phytochemicals of Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz. (PPY) and Paris fargesii Franch. (PF) in vitro and in vivo, and uncover their underlying mechanisms.
MATERIAL AND METHODS: MTT assays detected the cytotoxicity of extracts, active fractions, and active ingredients. To assess the suppressive effect on EC cells, Transwell and Scratch assays were employed. While Western blot assays and xenograft-bearing mouse models were utilized to explore the mechanism and the anti-metastasis effects in vitro and vivo.
RESULTS: Both extracts of PPY and PF, their active fractions, and ten principal ingredients all exhibited significant cytotoxicity against EC109 and KYSE520 cells. Furthermore, the two active fractions and their shared unique steroidal glycoside 8 inhibited cell migration via modulating TGF-β RI/Smad and TGF-β RI/RhoA/ROCK1 pathways and suppressed lung and liver metastases in vivo.
CONCLUSION: This study represents the first comprehensive exploration into the anti-metastatic potential of active fractions of PPY and PF, and their unique pennogenin glycoside 8 against EC in vitro and in vivo by modulating TGF-β RI/Smad and TGF-β RI/RhoA/ROCK1 pathways, highlighting their potential as therapeutic agents against EC metastasis.
AIM OF THE STUDY: This study aims to explore the anti-metastatic effects of the active fractions and phytochemicals of Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz. (PPY) and Paris fargesii Franch. (PF) in vitro and in vivo, and uncover their underlying mechanisms.
MATERIAL AND METHODS: MTT assays detected the cytotoxicity of extracts, active fractions, and active ingredients. To assess the suppressive effect on EC cells, Transwell and Scratch assays were employed. While Western blot assays and xenograft-bearing mouse models were utilized to explore the mechanism and the anti-metastasis effects in vitro and vivo.
RESULTS: Both extracts of PPY and PF, their active fractions, and ten principal ingredients all exhibited significant cytotoxicity against EC109 and KYSE520 cells. Furthermore, the two active fractions and their shared unique steroidal glycoside 8 inhibited cell migration via modulating TGF-β RI/Smad and TGF-β RI/RhoA/ROCK1 pathways and suppressed lung and liver metastases in vivo.
CONCLUSION: This study represents the first comprehensive exploration into the anti-metastatic potential of active fractions of PPY and PF, and their unique pennogenin glycoside 8 against EC in vitro and in vivo by modulating TGF-β RI/Smad and TGF-β RI/RhoA/ROCK1 pathways, highlighting their potential as therapeutic agents against EC metastasis.