Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control · 2016
[Investigation of two blood parasitic protozoa infection in farmed Macaca fascicularis in Guangxi Zhuang Autonomous Region].
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Yuan-Yuan Lu, Heng Peng, Huai-Min Zhu, Jian Li, Shao-Li Xue
Аннотация
OBJECTIVE: To investigate the infection situation of blood parasitic protozoa in farmed Macaca fascicularis in an animal breeding ground in Nanning, Guangxi Zhuang Autonomous Region, so as to provide the evidence for the prevention and control of human blood parasitic protozoa.
METHODS: A total of 993 blood samples from farmed M. fascicularis were collected and stored on FTA cards. Among them, 550 thin blood smears were made. Each 10 samples were mixed in groups, and then the Babesia spp. and Plasmodium spp. in the blood of M. fascicularis were detected by Nest-PCR and PCR, respectively. The positive groups were tested individually. The thin blood smears stained with Giemsa were examined microscopically when PCR reported the samples were positive.
RESULTS: When detected by Nest-PCR, the positive rate of Babesia. microti was 6.95% (69/993); only 1 positive sample with Plasmodium inui was detected by PCR. Among the 22 positive thin blood smears detected by PCR, 16 were determined with B. microti by microscopic examinations, on which the ring forms could be observed in the erythrocytes, but no hemozoin.
CONCLUSIONS: The positive rate of B. microti in M. fascicularis in Guangxi Zhuang Autonomous Region is high, and the animal may play a role as a reservoir host in the transmission of B. microti. In the screening of B. microti with low infection density, Nest-PCR has a higher sensitivity.
METHODS: A total of 993 blood samples from farmed M. fascicularis were collected and stored on FTA cards. Among them, 550 thin blood smears were made. Each 10 samples were mixed in groups, and then the Babesia spp. and Plasmodium spp. in the blood of M. fascicularis were detected by Nest-PCR and PCR, respectively. The positive groups were tested individually. The thin blood smears stained with Giemsa were examined microscopically when PCR reported the samples were positive.
RESULTS: When detected by Nest-PCR, the positive rate of Babesia. microti was 6.95% (69/993); only 1 positive sample with Plasmodium inui was detected by PCR. Among the 22 positive thin blood smears detected by PCR, 16 were determined with B. microti by microscopic examinations, on which the ring forms could be observed in the erythrocytes, but no hemozoin.
CONCLUSIONS: The positive rate of B. microti in M. fascicularis in Guangxi Zhuang Autonomous Region is high, and the animal may play a role as a reservoir host in the transmission of B. microti. In the screening of B. microti with low infection density, Nest-PCR has a higher sensitivity.