Chemiluminescent detection of glycocode alterations in hepatic granulomatous lesions of experimental schistosomiasis.

This work describes the glycophenotype evaluation of mice liver granulomatous lesion caused by infection of Schistosoma mansoni by using lectins labeled to acridinium ester (AE). The employed lectins were Concanavalin A (Con A), wheat germ agglutinin (WGA) and Sambucus nigra agglutinin (SNA) that specifically recognize α-D-glucose/mannose, N-acetyl-D-glucosamine and α-NeuNAc-[2→6]-Gal/GalNAc. The chemiluminescence expressed in relative light unit (RLU) obtained from the hepatic granuloma tissues (0.25 cm2) treated with the lectins-AE was compared with control tissues. Con A-AE infected tissues showed higher statistically significant values (1,501,182 ± 163,450 RLU) compared with the control tissue (575,280 ± 97,216 RLU). WGA-AE results also showed higher values (189,654 ± 20,686 RLU) than that found for the controls (82,878 ± 24,411). SNA-AE results did not present statistical difference between granulomatous tissues (198,990 ± 15,131) and controls (167,290 ± 25,194). There is a significant increase in glucose/mannose residues and N-acetyl-D-glucosamine in hepatic granuloma caused by S. mansoni, while the sialic acid remains virtually unchanged. The understanding of schistosome glycophenotype is relevant for the development of new diagnostic methods for schistosomiasis, design of new drug targets and preparation of glycan-based vaccines.