hsa-miR-144-3p and hsa-miR-4262 are exosomal biomarkers and function as tumor suppressors in HCMV positive brain tumor.

PURPOSE: The present retrospective observational study was designed to explore the expression deregulation of exosomal microRNAs, hsa-miR-144-3p, hsa-miR-4262, and related genes in brain tumor patients.

METHODS

Exosomes were extracted from 400 brain tumor patients and 400 controls and characterized using DLS, TEM, and ELISA. Quantitative PCR was used to check the expression level of hsa-miR-144-3p, hsa-miR-4262, phosphatidylinositol-3-kinase (PI3K) and AKT kinase (AKT) in study cohort. CMV level in brain tumor patients was checked by quantifying UL83.

RESULTS

Data analysis showed significant deregulation of hsa-miR-144-3p (p < 0.0001), hsa-miR-4262 (p < 0.0001), PI3K (p < 0.0001), and AKT (p < 0.0001) in brain tumor patients.

Conclusions

ROC curve analysis showed that deregulation of hsa-miR-144-3p, hsa-miR-4262, PI3K, and AKT may act as good diagnostic markers for brain tumor patients.