Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
Extracellular Vesicular Analysis of Glypican 1 mRNA and Protein for Pancreatic Cancer Diagnosis and Prognosis.
СтатьяПоддержка исследования: вне правительства СШАПоддержка NIH: внешняя программаНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Li Hong, Chiang Chi-Ling, Kwak Kwang Joo, Wang Xinyu, Doddi Sital, Ramanathan Lakshmi V, Cho Sun M, Hou Ya-Chin, Cheng Tai-Shan, Mo Xiaokui, Chang Yueh-Shih, Chang Hui-Lan, Cheng Weiming, Tsai Wei-Ni, Nguyen Luong T H, Pan Junjie, Ma Yifan, Rima Xilal Y, Zhang Jingjing, Reategui Eduardo, Chu Yeh-Shiu, Chang Peter Mu-Hsin, Chang Pei-Hung, Huang Chi-Ying F, Wang Cheng-Hsu, Shan Yan-Shen, Li Chung-Pin, Fleisher Martin, Lee L James
Аннотация
Detecting pancreatic duct adenocarcinoma (PDAC) in its early stages and predicting late-stage patient prognosis undergoing chemotherapy is challenging. This work shows that the activation of specific oncogenes leads to elevated expression of mRNAs and their corresponding proteins in extracellular vesicles (EVs) circulating in blood. Utilizing an immune lipoplex nanoparticle (ILN) biochip assay, these findings demonstrate that glypican 1 (GPC1) mRNA expression in the exosomes-rich (Exo) EV subpopulation and GPC1 membrane protein (mProtein) expression in the microvesicles-rich (MV) EV subpopulation, particularly the tumor associated microvesicles (tMV), served as a viable biomarker for PDAC. A combined analysis effectively discriminated early-stage PDAC patients from benign pancreatic diseases and healthy donors in sizable clinical from multiple hospitals. Furthermore, among late-stage PDAC patients undergoing chemotherapy, lower GPC1 tMV-mProtein and Exo-mRNA expression before treatment correlated significantly with prolonged overall survival. These findings underscore the potential of vesicular GPC1 expression for early PDAC screenings and chemotherapy prognosis.