Premature separation of centromeres in marrow chromosomes from an untreated patient with acute myelogenous leukemia.

Cytogenetic studies in an untreated patient with acute myelogenous leukemia revealed that greater than 60% of his marrow metaphases had pronounced anomalies in the centromere region. In the least affected chromosomes, the centromere appeared as a nonstaining hole, whereas in the most affected chromosomes, it appeared that the centromere segment had prematurely migrated at right angles to the telomeric portions. The population of mitoses exhibiting premature separation of the centromeres was reduced to virtually none during periods of drug induced remission. Our findings suggest that cytogenetic changes in malignant myeloid cells may be characterized by defects in centromere separation, as well as by alternations in karyotype.