Multi-Parameter Spectral Flow Cytometry Panel for Immune Phenotyping of Murine B and T Cell Responses.

To enable the analysis of B cell and T cell immune responses in mouse lymph nodes, spleen, and bone marrow, including different B cell and T cell subsets and their activation status, as well as the antigen-reactivity and isotype of B cells, a novel 31-parameter spectral flow cytometry panel was developed.