Iranian journal of immunology : IJI · 2025
Soluble HLA-E and Gastroesophageal Reflux Disease: A Novel Association.
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Al-Khalidy Huda Saleem Hantoosh Hameed, Salih Wafaa Hazim, Mahdi Batool Mutar
Аннотация
BACKGROUND: Gastroesophageal reflux disease (GERD) is a prevalent clinical condition that affects millions of individuals worldwide..
OBJECTIVE: To assess the level of soluble HLA-E (sHLA-E) as a biomarker in the diagnosis and immunopathogenesis of GERD patients..
METHODS: The case-control prospective study included 40 GERD patients who were consulted at the Gastroenterology Unit of Al-Kindy Teaching Hospital, as along with 40 healthy control subjects. The study period extended from January 2023 to May 2024. Blood was drawn from both groups and serum was separated to assesss HLA-E using a sandwich enzyme-linked immunosorbent assay (ELISA) kit..
RESULTS: There was a statistically significant difference in sHLA-E levels between GERD patients and healthy controls (p = 0.021). The median sHLA-E level was significantly higher in GERD patients (0.370 ng/mL) compared to controls (0.148 ng/mL). A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic performance of soluble HLA-E (sHLA-E) in predicting GERD. The area under the ROC curve (AUC) was calculated to assess the discriminatory ability of sHLA-E, with a value of 0.649 (95% CI: 0.534-0.752, p = 0.021). The optimal cutoff value for sHLA-E was determined to be ≤0.65 ng/mL, with a sensitivity of 85.1%, specificity of 27.3%, positive predictive value of 65.9%, negative predictive value of 69.4%, and accuracy of 35.0%..
CONCLUSION: The study provides evidence of an association between elevated sHLA-E levels and GERD. It also suggests that sHLA-E has a moderate discriminatory ability as a biomarker in predicting GERD..
OBJECTIVE: To assess the level of soluble HLA-E (sHLA-E) as a biomarker in the diagnosis and immunopathogenesis of GERD patients..
METHODS: The case-control prospective study included 40 GERD patients who were consulted at the Gastroenterology Unit of Al-Kindy Teaching Hospital, as along with 40 healthy control subjects. The study period extended from January 2023 to May 2024. Blood was drawn from both groups and serum was separated to assesss HLA-E using a sandwich enzyme-linked immunosorbent assay (ELISA) kit..
RESULTS: There was a statistically significant difference in sHLA-E levels between GERD patients and healthy controls (p = 0.021). The median sHLA-E level was significantly higher in GERD patients (0.370 ng/mL) compared to controls (0.148 ng/mL). A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic performance of soluble HLA-E (sHLA-E) in predicting GERD. The area under the ROC curve (AUC) was calculated to assess the discriminatory ability of sHLA-E, with a value of 0.649 (95% CI: 0.534-0.752, p = 0.021). The optimal cutoff value for sHLA-E was determined to be ≤0.65 ng/mL, with a sensitivity of 85.1%, specificity of 27.3%, positive predictive value of 65.9%, negative predictive value of 69.4%, and accuracy of 35.0%..
CONCLUSION: The study provides evidence of an association between elevated sHLA-E levels and GERD. It also suggests that sHLA-E has a moderate discriminatory ability as a biomarker in predicting GERD..