International journal of hematology · 2026
Ten-color multicolor flow cytometry-based measurable residual disease at pre-transplantation predicts relapse of acute myeloid leukemia: a prospective study.
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Tazoe Kumiyo, Nakamae Hirohisa, Makuuchi Yosuke, Kuno Masatomo, Takakuwa Teruhito, Okamura Hiroshi, Hirose Asao, Nakamae Mika, Nishimoto Mitsutaka, Nakashima Yasuhiro, Miyagawa Hideo, Hino Masayuki
Аннотация
OBJECTIVE: Measurable residual disease (MRD) is a key prognostic factor for outcomes following allogeneic hematopoietic stem cell transplantation (allo-HSCT) in patients with acute myeloid leukemia. To enhance predictive performance, we developed a combined MRD assessment method that integrates the proportion of residual leukemic cells of ten-color multicolor flow cytometry (MFC) with peripheral blood WT1 mRNA expression.
METHODS: Forty-six patients with acute myeloid leukemia who underwent allo-HSCT at our institution between October 2013 and December 2019 were enrolled. We prospectively analyzed the prognostic impact of ten-color MFC, peripheral blood WT1 mRNA, and combined MRD assessment before transplantation.
RESULTS: Both ten-color MFC-based MRD and peripheral blood WT1 mRNA levels above predefined thresholds were significantly associated with an increased risk of relapse after allo-HSCT. However, the results of the multivariate and ROC analyses suggest that ten-color MFC may be a stronger predictor than WT1 mRNA. Moreover, adding peripheral blood WT1 mRNA to ten-color MFC-based MRD reduced the predictive accuracy compared with MFC alone.
METHODS: Forty-six patients with acute myeloid leukemia who underwent allo-HSCT at our institution between October 2013 and December 2019 were enrolled. We prospectively analyzed the prognostic impact of ten-color MFC, peripheral blood WT1 mRNA, and combined MRD assessment before transplantation.
RESULTS: Both ten-color MFC-based MRD and peripheral blood WT1 mRNA levels above predefined thresholds were significantly associated with an increased risk of relapse after allo-HSCT. However, the results of the multivariate and ROC analyses suggest that ten-color MFC may be a stronger predictor than WT1 mRNA. Moreover, adding peripheral blood WT1 mRNA to ten-color MFC-based MRD reduced the predictive accuracy compared with MFC alone.