Медицинская библиотека · World journal of pediatrics : WJP · 2019

Attenuated MuV-S79 as vector stably expressing foreign gene.

Zhou Duo, Zhu Meng-Ying, Wang Yi-Long, Hao Xiao-Qiang, Zhou Dong-Ming, Liu Rong-Xian, Zhang Chu-Di, Qu Chu-Fan, Zhao Zheng-Yan

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Аннотация

BACKGROUND

To describe mumps virus (MuV) used as a vector to express enhanced green fluorescent protein (EGFP) or red fluorescent protein (RFP) genes.

METHODS

Molecular cloning technique was applied to establish the cDNA clones of recombinant mumps viruses (rMuVs). rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity.

RESULTS

Three recombinant viruses replicated well in Vero cells and similarly as parental rMuV-S79, expressed heterologous genes in high levels, and were genetically stable in at least 15 passages.

CONCLUSION

rMuV-S79 is a promising platform to accommodate foreign genes like marker genes, other antigens and immunomodulators for addressing various diseases.

Для цитирования: Zhou Duo, Zhu Meng-Ying, Wang Yi-Long, Hao Xiao-Qiang, Zhou Dong-Ming, Liu Rong-Xian, Zhang Chu-Di, Qu Chu-Fan, Zhao Zheng-Yan. Attenuated MuV-S79 as vector stably expressing foreign gene.. World journal of pediatrics : WJP. 2019. DOI: 10.1007/s12519-019-00287-7.

Предметные рубрики MeSH

Animals Chlorocebus aethiops Cloning, Molecular Green Fluorescent Proteins Luminescent Proteins Mumps virus Reverse Genetics Vero Cells Red Fluorescent Protein

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