The mode of action of splenic suppressor cells in murine plasmacytoma.

Polyclonal IgM secretion after LPS stimulation and antigen-dependent antibody production by splenic cells of BALB/c mice with plasmacytoma were found to be markedly reduced. The former occurred without mediation of suppressor cells, probably as the result of a quantitative reduction of mitogen-responsive B lymphocytes. On the other hand, the reduction in the generation of antibodies to sheep erythrocytes in vitro, as determined by the number of PFC, was found to be dependent on the activity of suppressor cells which had the functional characteristics of macrophages. The targets of these suppressor cells were found to be normal macrophages, B and T lymphocytes which were all independently suppressed. A diffusible factor appeared to be responsible for the suppressive effect of the suppressor macrophages interfering mainly with those early stages of the immune response involving antigen-triggered B cell activation.