Investigating Bacterial Invasion of Highly Differentiated Human Bronchial Epithelial Cell Barriers.

Confocal microscopy provides information on host-pathogen interactions. To examine infection at the respiratory epithelial barrier, primary bronchial cells differentiated at an air-liquid interface (ALI) can be imaged and reconstructed as a 3D model. In this chapter, we describe a method for immunostaining and fluorescence imaging of well-differentiated bronchial epithelial tissue that has been exposed to the cystic fibrosis respiratory pathogen Achromobacter xylosoxidans.