Gut microbes · 2025
Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Morsy Yasser, Walberg Åsa, Wawrzyniak Paulina, Hubeli Barbara, Truscello Luca, Mamie Celine, Niechcial Ania, Gueguen Emilie, Manzini Roberto, Gottier Claudia, Lang Silvia, Scharl Sylvie, Blümel Sena, Biedermann Luc, Rogler Gerhard, Turina Matthias, Ramser Michaela, Petrowsky Henrik, Arnold Isabelle C, Zeissig Sebastian, Zamboni Nicola, Egli Adrian, Niess Jan Hendrik, Hruz Petr, Knuth Alexander, Fritsch Ralph, Manz Markus G, Wawrzyniak Marcin, Scharl Michael
Аннотация
The involvement of the intestinal microbiome in the pathogenesis of inflammatory bowel disease (IBD) and colorectal cancer (CRC), is well-established. Bacteria interact with immune cells at sites of intestinal inflammation, but also in the CRC tumor microenvironment. We hypothesized that bacterial remnants translocate within peripheral blood mononuclear cells (PBMCs) into the circulation and thus explored the composition of the detectable microbiome in PBMCs of patients with CRC or IBD compared to healthy controls. The PBMC microbiome profiles partially align with the tumor-derived or intestinal tissue-derived microbiome signatures obtained from the same patients with CRC or IBD, respectively. Our metagenomics data, supported by 16S-rRNA-FISH-Flow, imaging flow cytometry and species-specific qPCR, revealed the presence of translocated bacterial genetic sequences in the patients with CRC and IBD. Thus, our data suggest that in patients with intestinal barrier leakage, there is the potential for the translocation of bacterial remnants into the circulation via PBMCs.