Cytogenetic abnormalities in a human null cell leukemia line (REH).

The chromosomes of a null cell line (REH6) derived from peripheral leukocytes of a patient with acute lymphoid leukemia (ALL) was examined with conventional and R-banding techniques on fresh and established cells bearing ALL-associated antigen(s). Five marker chromosomes were found in both fresh and established cells. The constitution of these abnormalities is related to and explained by breaks and translocations involving five chromosomes. Furthermore, one X chromosome is completely missing. The modal chromosome number in vitro was 45 up to 10 months, and 46 from 10 to 43 months after establishment of the cell line.