[M-29 human diploid cells line--a substrate for the production of antiviral vaccines].

AIM: Study of morphologic and karyologic characteristics of 5 russian human diploid cell lines (HDC).

MATERIALS AND

METHODS

5 HDC lines and HDC strain MRC-5 were studied; RK-13 and Vero continuous cell lines were used; viruses: rubella (RA27/3), measles (L-16), epidemic parotitis (L-3). Cytogenetic analysis of HDC was performed by using DAPI differential staining method.

RESULTS

M-29 line has characteristics that are similar to those of MRC-5 diploid cell strain. M-29 cell culture is not contaminated with foreign viruses, mycoplasmata, does not have oncogenic potency.

CONCLUSION

M-29 line has high virus-productive properties for accumulation of measles, rubella and epidemic parotitis vaccine viruses and may be recommended as a substrate for the production of antiviral vaccines.