The Journal of veterinary medical science · 1999
Biological activities of lipopolysaccharides extracted from porcine vaccine strains.
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Ogikubo Y, Norimatsu M, Kojima A, Sasaki Y, Tamura Y
Аннотация
Lipopolysaccharides (LPSs) were purified from Actinobacillus pleuropneumoniae serotype 2, Bordetella bronchiseptica and Haemophilus parasuis serotype 5, which were used for vaccine production in Japan, by the phenol-water procedure. In SDS-PAGE analysis, A. pleuropneumoniae LPS, as well as Escherichia coli LPS, demonstrated a typical ladder profile of a smooth-type LPS. On the other hand, B. bronchiseptica and H. parasuis LPSs lacked the ladder profiles. It was found that the biological activity of these LPSs was comparable to those of E. coli LPS in terms of activation of the clotting enzyme of Limulus amoebocyte lysate, mitogenic activity of mouse spleen cells, stimulation of TNF-alpha and nitric oxide production, but IL-6 production could hardly be observed in any LPS.
Предметные рубрики MeSH
Actinobacillus InfectionsActinobacillus pleuropneumoniaeAnimalsBacterial VaccinesBordetella InfectionsBordetella bronchisepticaElectrophoresis, Polyacrylamide GelFormazansHaemophilusHaemophilus InfectionsHaemophilus VaccinesInterleukin-6Lethal Dose 50Limulus TestLipopolysaccharidesMiceMice, Inbred BALB CNitric OxideSwineSwine DiseasesTetrazolium SaltsTumor Necrosis Factor-alphaVaccination