Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials · 2014
[Influence of huatanjiangqi prescription on multidrug resistance-associated protein 1 in human bronchial epithelial cells].
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Wang Chen-Yin, Wang Dian-Lei, Tao Xiu-Hua, Yang Li-Li, Chen Jin-Pei, Cao Yin
Аннотация
OBJECTIVE: To investigate the effect of Huatanjiangqi prescription (Sinapis Semen, Perillae Fructus, Cynanchi Stauntonii Rhizoma et Radix, Inulae Herba, Angelicae Sinensis Radix, Honey-fried Ephedrae Herba) on multidrug resistance-associated protein 1 (MRP1) in human bronchial epithelial cells.
METHODS: The human bronchial epithelial cells line 16HBE140- was used to analyze the in vitro effect of Huatanjiangqi prescription on MRP1 transport. 5-CFDA was used as a model MRP1 substrate and was measured with flow cytometry. The mRNA expression of MRP1 was detected by real-time PCR.
RESULTS: Huatanjiangqi prescription could promote the proliferation of human bronchial epithelial cells 16HBE140- in a certain range of concentration; Compared with the control group (5-CFDA), low, medium and high concentration (100, 1 000, 2 000 microg/mL) of Huatanjiangqi prescription on MRP1 function were increased by 22.59%, 47.14% and 68.36%, respectively; Huatanjiangqi prescription could concentration-dependently induce the expression of MRP1 mRNA, medium and high concentration could induce a significant difference.
CONCLUSION: Huatanjiangqi prescription can improve MRP1 efflux function and mRNA levels in a concetration-dependent manner.
METHODS: The human bronchial epithelial cells line 16HBE140- was used to analyze the in vitro effect of Huatanjiangqi prescription on MRP1 transport. 5-CFDA was used as a model MRP1 substrate and was measured with flow cytometry. The mRNA expression of MRP1 was detected by real-time PCR.
RESULTS: Huatanjiangqi prescription could promote the proliferation of human bronchial epithelial cells 16HBE140- in a certain range of concentration; Compared with the control group (5-CFDA), low, medium and high concentration (100, 1 000, 2 000 microg/mL) of Huatanjiangqi prescription on MRP1 function were increased by 22.59%, 47.14% and 68.36%, respectively; Huatanjiangqi prescription could concentration-dependently induce the expression of MRP1 mRNA, medium and high concentration could induce a significant difference.
CONCLUSION: Huatanjiangqi prescription can improve MRP1 efflux function and mRNA levels in a concetration-dependent manner.
Предметные рубрики MeSH
BronchiCell LineCell ProliferationDose-Response Relationship, DrugDrug CombinationsDrugs, Chinese HerbalEpithelial CellsFlow CytometryFluoresceinsHumansATP-Binding Cassette, Sub-Family C ProteinsPlants, MedicinalProtein TransportPulmonary Disease, Chronic ObstructiveRNA, MessengerReal-Time Polymerase Chain Reaction