[Establishment of one-step reverse transcription-polymerase chain reaction method for mumps virus hydrophobin gene detection].

OBJECTIVE

To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).

METHODS

To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.

RESULTS

At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.

CONCLUSIONS

The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.