The Fading Art of Microsphere-Derived Measurement of Absolute Myocardial Blood Flow.

The measurement of absolute myocardial blood flow (MBF) has played a pivotal role in the development of nuclear cardiology and other perfusion imaging techniques. However, the capacity to perform such experiments may be diminished. This review examines the basic physiology of microsphere measurement of absolute MBF which was developed over 50 years ago, with multiple refinements over time. The use of different types of microspheres is presented in depth. The set-up and performance of a large animal model is detailed with tips and pitfalls explained. It is the purpose of this review to stimulate the next generation of investigators into considering this skill as part of their research tool box.