Wei sheng yan jiu = Journal of hygiene research · 2021
[Comparative study of the detection of serum folate with improved microbial assay and electrochemiluminescence method].
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Li Yan, Wang Zhu, Mao Hongmei, Wang Jingbo, Gong Weiyi, Chen Chen, Shi Lili, Sun Jing, Huo Junsheng
Аннотация
OBJECTIVE: To compare the result of serum folate determined by improved microbial assay and electrochemiluminescence method, and to look for the relationship between them, so as to provide basis for the assessment of nutrition status of folate in population.
METHODS: A total of 258 serum samples were examined by improved microbial assay and electrochemiluminescence method. The correlation and consistence of the two method were analyzed.
RESULTS: The result showed that the correlation coefficient of the two method was 0. 885, which indicated that the result of two method were highly correlated. Results of Bland-Altman method showed that 94. 5% of the values were within the consistency limit, and the Kappa value of Kappa test was 0. 665. The result of consistency analysis showed that there were some differences between the two methods, and the result of serum folate tested by improved microbial assay were higher than that of electrochemiluminescence method in general.
CONCLUSION: The result of serum folate tested by electrochemiluminescence were highly correlated with the improved microbial assay, yet there are some differences in the consistency result between the two methods. Evaluating the nutrition status of folate by electrochemiluminescence may lead to a higher number of folate deficiency.
METHODS: A total of 258 serum samples were examined by improved microbial assay and electrochemiluminescence method. The correlation and consistence of the two method were analyzed.
RESULTS: The result showed that the correlation coefficient of the two method was 0. 885, which indicated that the result of two method were highly correlated. Results of Bland-Altman method showed that 94. 5% of the values were within the consistency limit, and the Kappa value of Kappa test was 0. 665. The result of consistency analysis showed that there were some differences between the two methods, and the result of serum folate tested by improved microbial assay were higher than that of electrochemiluminescence method in general.
CONCLUSION: The result of serum folate tested by electrochemiluminescence were highly correlated with the improved microbial assay, yet there are some differences in the consistency result between the two methods. Evaluating the nutrition status of folate by electrochemiluminescence may lead to a higher number of folate deficiency.