Fertility and sterility · 2009
Separation of a pronucleus by premature cytokinesis: a mechanism for immediate diploidization of tripronuclear oocytes?
Клинический случайСтатьяНазвание и аннотация: Перевод в очереди
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Rosenbusch Bernd E, Schneider Michael
Аннотация
OBJECTIVE: To describe an oocyte with a peculiar combination of abnormalities in terms of cytoplasmic fragmentation and formation of pronuclei.
DESIGN: Case report.
SETTING: University-based IVF unit.
PATIENT(S): A chromosomally normal couple undergoing the third treatment by assisted reproduction.
INTERVENTION(S): Controlled ovarian hyperstimulation, follicular aspiration, testicular sperm extraction, intracytoplasmic sperm injection (ICSI), and oocyte fixation.
MAIN OUTCOME MEASURE(S): Assessment of pronuclear formation and cytogenetic analysis of a tripronuclear fragmented oocyte.
RESULT(S): The described oocyte revealed two polar body-like structures after follicular aspiration and developed three pronuclei after ICSI, accompanied by extrusion of another polar body-like globule. Moreover, the pronuclear stage had undergone a premature irregular cytokinesis, including one of the pronuclei in the smaller half of the divided cytoplasm. Cytogenetic analysis showed a triploid karyotype (23,X/23,X,ace/23,Y) commonly found in digynic ICSI zygotes.
CONCLUSION(S): The chromosomal constitution suggests that the third globule represents another cytoplasmic fragment and no regular second polar body. Separation of a supernumerary pronucleus by premature cytokinesis might be relevant for the discussion on diploidization of triploid zygotes.
DESIGN: Case report.
SETTING: University-based IVF unit.
PATIENT(S): A chromosomally normal couple undergoing the third treatment by assisted reproduction.
INTERVENTION(S): Controlled ovarian hyperstimulation, follicular aspiration, testicular sperm extraction, intracytoplasmic sperm injection (ICSI), and oocyte fixation.
MAIN OUTCOME MEASURE(S): Assessment of pronuclear formation and cytogenetic analysis of a tripronuclear fragmented oocyte.
RESULT(S): The described oocyte revealed two polar body-like structures after follicular aspiration and developed three pronuclei after ICSI, accompanied by extrusion of another polar body-like globule. Moreover, the pronuclear stage had undergone a premature irregular cytokinesis, including one of the pronuclei in the smaller half of the divided cytoplasm. Cytogenetic analysis showed a triploid karyotype (23,X/23,X,ace/23,Y) commonly found in digynic ICSI zygotes.
CONCLUSION(S): The chromosomal constitution suggests that the third globule represents another cytoplasmic fragment and no regular second polar body. Separation of a supernumerary pronucleus by premature cytokinesis might be relevant for the discussion on diploidization of triploid zygotes.