Clinical laboratory · 2023
Molecular Evaluation of β-Lactamase (blaSHV, blaTEM, blaCTX-M) Genes in Multidrug Resistant of Klebsiella Pneumoniae.
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Nargesian Mansooreh, Saffari Mahmood, Zargar Mohsen
Аннотация
BACKGROUND: Klebsiella is an opportunistic pathogen, which is the most common causes of nosocomial infections. To date, the prevalence of ESBL-producing pathogens has increased and is associated with mortality, morbidity, and healthcare costs. The aim of this investigation was to determine the frequency of blaSHV, blaTEM, and blaCTX-M genes from Klebsiella pneumonia isolated from patients with UTI in the city of Qom.
METHODS: In the cross-sectional study, a total of 500 urinary samples were cultured in MacConkey agar and identified using the biochemical test. For a total of 340 positive K. pneumonia samples the antimicrobial susceptibility was determined using the Kirby-Bauer disc diffusion approach. For molecular genotyping, the frequencies of blaSHV, blaCTX-M, and blaTEM genes were determined using a polymerase chain reaction (PCR) method.
RESULTS: Our finding revealed that a total of 340 K. pneumonia isolates 110 isolates (32.35%) were ESBL producers by the phenotypic method. All of these isolates were assessed by PCR for blaSHV, blaCTX-M, and blaTEM genes. The PCR results demonstrated that the frequencies of blaTEM, blaCTX-M, and blaSHV genes were 59.09% (65 isolates), 74.54% (82 isolates), and 74.54% (82 isolates), respectively.
CONCLUSIONS: According to our findings, with the higher prevalence of ESBL-producing isolates in the clinical, early detection, and follow-up procedures are critical strategies to the prevention of the spread of multidrug resistant isolates.
METHODS: In the cross-sectional study, a total of 500 urinary samples were cultured in MacConkey agar and identified using the biochemical test. For a total of 340 positive K. pneumonia samples the antimicrobial susceptibility was determined using the Kirby-Bauer disc diffusion approach. For molecular genotyping, the frequencies of blaSHV, blaCTX-M, and blaTEM genes were determined using a polymerase chain reaction (PCR) method.
RESULTS: Our finding revealed that a total of 340 K. pneumonia isolates 110 isolates (32.35%) were ESBL producers by the phenotypic method. All of these isolates were assessed by PCR for blaSHV, blaCTX-M, and blaTEM genes. The PCR results demonstrated that the frequencies of blaTEM, blaCTX-M, and blaSHV genes were 59.09% (65 isolates), 74.54% (82 isolates), and 74.54% (82 isolates), respectively.
CONCLUSIONS: According to our findings, with the higher prevalence of ESBL-producing isolates in the clinical, early detection, and follow-up procedures are critical strategies to the prevention of the spread of multidrug resistant isolates.