Virology journal · 2008
Genetic heterogeneity of L-Zagreb mumps virus vaccine strain.
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Kosutic-Gulija Tanja, Forcic Dubravko, Santak Maja, Ramljak Ana, Mateljak-Lukacevic Sanja, Mazuran Renata
Аннотация
BACKGROUND: The most often used mumps vaccine strains Jeryl Lynn (JL), RIT4385, Urabe-AM9, L-Zagreb and L-3 differ in immunogenicity and reactogenicity. Previous analyses showed that JL, Urabe-AM9 and L-3 are genetically heterogeneous.
RESULTS: We identified the heterogeneity of L-Zagreb throughout the entire genome. Two major variants were defined: variant A being identical to the consensus sequence of viral seeds and vaccine(s) and variant B which differs from variant A in three nucleotide positions. The difference between viral variants in L-Zagreb strain is insufficient for distinct viral strains to be defined. We demonstrated that proportion of variants in L-Zagreb viral population depends on cell substrate used for viral replication in vitro and in vivo.
CONCLUSION: L-Zagreb strain should be considered as a single strain composed of at least two variant viral genomes.
RESULTS: We identified the heterogeneity of L-Zagreb throughout the entire genome. Two major variants were defined: variant A being identical to the consensus sequence of viral seeds and vaccine(s) and variant B which differs from variant A in three nucleotide positions. The difference between viral variants in L-Zagreb strain is insufficient for distinct viral strains to be defined. We demonstrated that proportion of variants in L-Zagreb viral population depends on cell substrate used for viral replication in vitro and in vivo.
CONCLUSION: L-Zagreb strain should be considered as a single strain composed of at least two variant viral genomes.
Предметные рубрики MeSH
AnimalsCell LineCell Line, TumorChick EmbryoChlorocebus aethiopsCroatiaFibroblastsGenetic VariationGenome, ViralHumansMumps VaccineMumps virusPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthReverse Transcriptase Polymerase Chain ReactionSpecies SpecificityVero CellsVirus CultivationVirus Replication